PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS

Photobiomodulatory effects of low-intensity laser radiation (LILR) in cells cultured standard, two-dimensional conditions are well established. Conversely, the characteristics this effect three-dimensional (3D) cultures, which currently recommended due to greater similarity with cellular behavior vivo, have not yet been widely investigated. The objective work was analyze biomodulator LILR, on wavelength (λ) 685 nm, constitution process and viability as multicellular spheroid (MSs). For this, agarose molds containing microwells were seeded (2x105 cell/ml) osteogenic precursor (OPCs - MC3T3-E1) kept under ideal culture conditions. irradiated for five consecutive days doses 0.5, 1.0 1.5 J/cm², first irradiation being performed immediately after sowing. MSs analyzed cultures submitted cell test. results demonstrated that LILR at λ nm exerted a dose-dependent biomodulatory metabolism constituting OPCs. These demonstrate potential photobiomodulation contribute MSs, can be explored strategies spheroids therapy used regenerative medicine bioprinting.